The growing demand for recombinant proteins has led to exploring new engineering strategies and the expansion of protein expression host cell lines. However, this endeavor is not for the faint of heart. Many variables must be considered during the development process, including verification and sequence analysis of the gene or protein of interest, codon optimization, vector construction and clone/host selection. When challenges arise, protein expression scientists must design new cloning schemes by altering the DNA or amino acid sequence, reassigning the codon to another, moving a gene for one vector to another, transfecting the vector to an alternative host, re-selecting the clone, re-characterizing the expressed protein or any of the above – a laborious, time consuming and expensive process. Cambridge Healthtech Institute's 6th Annual Cell Line and Systems Engineering conference features effective engineering strategies for recombinant protein expression and production that lead to functional protein products. Learn from seasoned, savvy researchers as they share their real-world experiences, applications, and results.